› 1s4p

X-ray diffraction
2.01Å resolution

Crystal structure of yeast alpha1,2-mannosyltransferase Kre2p/Mnt1p: ternary complex with GDP/Mn and methyl-alpha-mannoside acceptor

Released:
DOI: 10.2210/pdb1s4p/pdb
PDB entry 1s4p coloured by chain, front view.
PDB entry 1s4p coloured by chain, side view.
PDB entry 1s4p coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of Kre2p/Mnt1p: a yeast alpha1,2-mannosyltransferase involved in mannoprotein biosynthesis.
Lobsanov YD, Romero PA, Sleno B, Yu B, Yip P, Herscovics A, Howell PL
J Biol Chem 279 17921-31 (2004)
PMID: 14752117

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151077 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Glycolipid 2-alpha-mannosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 348 amino acids
Theoretical weight: 41.35 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P27809 (Residues: 97-442; Coverage: 78%)
Gene names: D8035.26, KRE2, MNT1, YDR483W
Sequence domains: Glycolipid 2-alpha-mannosyltransferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
5 bound ligands:
Ligand MMA 2 x MMA
Ligand MN 2 x MN
Ligand CL 4 x CL
Ligand GDP 2 x GDP
Ligand EPE 2 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21
Unit cell:
a: 60.694Å b: 101.068Å c: 62.036Å
α: 90° β: 98.81° γ: 90°
R-values:
R R work R free
0.203 0.203 0.256
Expression system: Komagataella pastoris

Quick links

Citations

10 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
9 more

4 mentions without citation

Structure of the Glycosyltransferase Ktr4p from Saccharomyces cerevisiae.
Possner et al. (2015)
3 more