› 1rvy

X-ray diffraction
2.9Å resolution

E75Q MUTANT OF RABBIT CYTOSOLIC SERINE HYDROXYMETHYLTRANSFERASE, COMPLEX WITH GLYCINE

Released:
DOI: 10.2210/pdb1rvy/pdb
PDB entry 1rvy coloured by chain, front view.
PDB entry 1rvy coloured by chain, side view.
PDB entry 1rvy coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Serine hydroxymethyltransferase: role of glu75 and evidence that serine is cleaved by a retroaldol mechanism.
Szebenyi DM, Musayev FN, di Salvo ML, Safo MK, Schirch V
Biochemistry 43 6865-76 (2004)
PMID: 15170323

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139606 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine hydroxymethyltransferase, cytosolic Chains: A, B
Molecule details ›
Chains: A, B
Length: 483 amino acids
Theoretical weight: 52.92 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P07511 (Residues: 2-484; Coverage: 100%)
Gene name: SHMT1
Sequence domains: Serine hydroxymethyltransferase
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
2 bound ligands:
Ligand PLG 1 x PLG
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P41212
Unit cell:
a: 114.7Å b: 114.7Å c: 156.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.22 0.283
Expression system: Escherichia coli

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Citations

3 review citations

Serine hydroxymethyltransferase revisited.
Schirch et al. (2005)
2 more