› 1rsm

X-ray diffraction
2Å resolution

THE 2-ANGSTROMS RESOLUTION STRUCTURE OF A THERMOSTABLE RIBONUCLEASE A CHEMICALLY CROSS-LINKED BETWEEN LYSINE RESIDUES 7 AND 41

Released:
DOI: 10.2210/pdb1rsm/pdb
PDB entry 1rsm coloured by chain, front view.
PDB entry 1rsm coloured by chain, side view.
PDB entry 1rsm coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
The 2-A resolution structure of a thermostable ribonuclease A chemically cross-linked between lysine residues 7 and 41.
Weber PC, Sheriff S, Ohlendorf DH, Finzel BC, Salemme FR
Proc Natl Acad Sci U S A 82 8473-7 (1985)
PMID: 3936036

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.71 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand NIN 1 x NIN
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 37.05Å b: 41.26Å c: 75.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.184 not available
Expression system: Not provided

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Citations

1 review citation

Protein Macrocyclization for Tertiary Structure Stabilization.
Haim et al. (2021)
7 other articles

1 mention without citation

Rapid catalytic template searching as an enzyme function prediction procedure.
Nilmeier et al. (2013)