› 1rmd

X-ray diffraction
2.1Å resolution

RAG1 DIMERIZATION DOMAIN

Released:
DOI: 10.2210/pdb1rmd/pdb
PDB entry 1rmd coloured by chain, front view.
PDB entry 1rmd coloured by chain, side view.
PDB entry 1rmd coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of the RAG1 dimerization domain reveals multiple zinc-binding motifs including a novel zinc binuclear cluster.
Bellon SF, Rodgers KK, Schatz DG, Coleman JE, Steitz TA
Nat Struct Biol 4 586-91 (1997)
PMID: 9228952

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147533 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
V(D)J recombination-activating protein 1 Chain: A
Molecule details ›
Chain: A
Length: 116 amino acids
Theoretical weight: 13.15 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P15919 (Residues: 265-380; Coverage: 11%)
Gene name: Rag1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P3221
Unit cell:
a: 57.58Å b: 57.58Å c: 98.43Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.209 0.257
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Keep your fingers off my DNA: protein-protein interactions mediated by C2H2 zinc finger domains.
Brayer et al. (2008)
19 more

17 mentions without citation

Riches in RAGs: Revealing the V(D)J Recombinase through High-Resolution Structures.
Rodgers KK. (2017)
16 more