› 1rl9

X-ray diffraction
1.45Å resolution

Crystal structure of Creatine-ADP arginine kinase ternary complex

Released:
DOI: 10.2210/pdb1rl9/pdb
PDB entry 1rl9 coloured by chain, front view.
PDB entry 1rl9 coloured by chain, side view.
PDB entry 1rl9 coloured by chain, top view.
Source organism: Limulus polyphemus
Primary publication:
The role of phosphagen specificity loops in arginine kinase.
Azzi A, Clark SA, Ellington WR, Chapman MS
Protein Sci 13 575-85 (2004)
PMID: 14978299

Function and Biology Details

Reaction catalysed: 
ATP + L-arginine = ADP + N(omega)-phospho-L-arginine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156357 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginine kinase Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 40.31 KDa
Source organism: Limulus polyphemus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P51541 (Residues: 1-357; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand ADP 1 x ADP
Ligand IOM 1 x IOM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 64.1Å b: 65.3Å c: 85.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.194 0.237
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

Relating structure to mechanism in creatine kinase.
McLeish et al. (2005)
5 more

4 mentions without citation

A tyrosine kinase and its activator control the activity of the CtsR heat shock repressor in B. subtilis.
Kirstein et al. (2005)
3 more