› 1rkt

X-ray diffraction
1.95Å resolution

Crystal structure of yfiR, a putative transcriptional regulator from Bacillus subtilis

Released:
DOI: 10.2210/pdb1rkt/pdb
PDB entry 1rkt coloured by chain, front view.
PDB entry 1rkt coloured by chain, side view.
PDB entry 1rkt coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structure of YfiR, an unusual TetR/CamR-type putative transcriptional regulator from Bacillus subtilis.
Rajan SS, Yang X, Shuvalova L, Collart F, Anderson WF
Proteins 65 255-7 (2006)
PMID: 16862575

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128307 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized HTH-type transcriptional regulator YfiR Chains: A, B
Molecule details ›
Chains: A, B
Length: 205 amino acids
Theoretical weight: 23.9 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O31560 (Residues: 1-205; Coverage: 100%)
Gene names: BSU08370, yfiR
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand UNX 39 x UNX
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P1
Unit cell:
a: 47.983Å b: 48.765Å c: 57.233Å
α: 71.96° β: 70.65° γ: 80.2°
R-values:
R R work R free
0.21 0.208 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Structures of AcrR and CmeR: insight into the mechanisms of transcriptional repression and multi-drug recognition in the TetR family of regulators.
Routh et al. (2009)
2 more

2 mentions without citation

The TetR family of transcriptional repressors.
Ramos et al. (2005)
1 more