› 1rhz

X-ray diffraction
3.5Å resolution

The structure of a protein conducting channel

Released:
DOI: 10.2210/pdb1rhz/pdb
PDB entry 1rhz coloured by chain, front view.
PDB entry 1rhz coloured by chain, side view.
PDB entry 1rhz coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
X-ray structure of a protein-conducting channel.
Van den Berg B, Clemons WM, Collinson I, Modis Y, Hartmann E, Harrison SC, Rapoport TA
Nature 427 36-44 (2004)
PMID: 14661030

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-158008 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein translocase subunit SecY Chain: A
Molecule details ›
Chain: A
Length: 436 amino acids
Theoretical weight: 47.48 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q60175 (Residues: 1-436; Coverage: 100%)
Gene names: MJ0478, secY
Sequence domains:
Structure domains: SecY subunit domain
Protein translocase subunit SecE Chain: B
Molecule details ›
Chain: B
Length: 74 amino acids
Theoretical weight: 8.45 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57817 (Residues: 1-74; Coverage: 100%)
Gene names: MJ0371, secE
Sequence domains: SecE/Sec61-gamma subunits of protein translocation complex
Structure domains: Preprotein translocase SecE subunit
Preprotein translocase subunit SecG Chain: C
Molecule details ›
Chain: C
Length: 53 amino acids
Theoretical weight: 5.97 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: P60460 (Residues: 1-53; Coverage: 100%)
Gene names: MJ0912.1, secG
Sequence domains: Sec61beta family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21212
Unit cell:
a: 92.746Å b: 149.361Å c: 79.475Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.254 0.254 0.33
Expression system: Escherichia coli

Quick links

Citations

208 review citations

The bacterial cell envelope.
Silhavy et al. (2010)
207 more

55 mentions without citation

Mechanisms of integral membrane protein insertion and folding.
Cymer et al. (2015)
54 more