› 1qzr

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF THE ATPASE REGION OF SACCHAROMYCES CEREVISIAE TOPOISOMERASE II BOUND TO ICRF-187 (DEXRAZOXANE)

Released:
DOI: 10.2210/pdb1qzr/pdb
PDB entry 1qzr coloured by chain, front view.
PDB entry 1qzr coloured by chain, side view.
PDB entry 1qzr coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of the topoisomerase II ATPase region and its mechanism of inhibition by the chemotherapeutic agent ICRF-187.
Classen S, Olland S, Berger JM
Proc Natl Acad Sci U S A 100 10629-34 (2003)
PMID: 12963818

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139226 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 418 amino acids
Theoretical weight: 48.1 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P06786 (Residues: 1-413; Coverage: 29%)
Gene names: N2244, TOP2, TOR3, YNL088W
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand ANP 2 x ANP
Ligand CDX 1 x CDX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 58.553Å b: 71.444Å c: 215.724Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.196 0.237
Expression system: Escherichia coli

Quick links

Citations

28 review citations

Targeting DNA topoisomerase II in cancer chemotherapy.
Nitiss JL. (2009)
27 more

12 mentions without citation

Topoisomerases as anticancer targets.
Delgado et al. (2018)
11 more