› 1qws

X-ray diffraction
1.9Å resolution

Structure of the D181N variant of catalase HPII from E. coli

Released:
DOI: 10.2210/pdb1qws/pdb
PDB entry 1qws coloured by chain, front view.
PDB entry 1qws coloured by chain, side view.
PDB entry 1qws coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
An electrical potential in the access channel of catalases enhances catalysis.
Chelikani P, Carpena X, Fita I, Loewen PC
J Biol Chem 278 31290-6 (2003)
PMID: 12777389

Function and Biology Details

Reaction catalysed: 
2 H(2)O(2) = O(2) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149122 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catalase HPII Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 753 amino acids
Theoretical weight: 84.27 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P21179 (Residues: 1-753; Coverage: 100%)
Gene names: JW1721, b1732, katE
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 4 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 93.434Å b: 132.881Å c: 122.098Å
α: 90° β: 109.66° γ: 90°
R-values:
R R work R free
0.164 0.164 0.22
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Evolution of catalases from bacteria to humans.
Zamocky et al. (2008)
9 more