› 1q05

X-ray diffraction
2.2Å resolution

Crystal structure of the Cu(I) form of E. coli CueR, a copper efflux regulator

Released:
DOI: 10.2210/pdb1q05/pdb
PDB entry 1q05 coloured by chain, front view.
PDB entry 1q05 coloured by chain, side view.
PDB entry 1q05 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Molecular basis of metal-ion selectivity and zeptomolar sensitivity by CueR.
Changela A, Chen K, Xue Y, Holschen J, Outten CE, O'Halloran TV, Mondragón A
Science 301 1383-7 (2003)
PMID: 12958362

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141874 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator CueR Chains: A, B
Molecule details ›
Chains: A, B
Length: 135 amino acids
Theoretical weight: 15.26 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A9G4 (Residues: 1-135; Coverage: 100%)
Gene names: JW0476, b0487, copR, cueR, ybbI
Sequence domains:
Structure domains: Multidrug-efflux Transporter Regulator; Chain: A; Domain 2

Ligands and Environments

1 bound ligand:
Ligand CU1 2 x CU1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P212121
Unit cell:
a: 59.16Å b: 66.26Å c: 81.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.253
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

70 review citations

Antimicrobial activity of metals: mechanisms, molecular targets and applications.
Lemire et al. (2013)
69 more

18 mentions without citation

Coordination chemistry of bacterial metal transport and sensing.
Ma et al. (2009)
17 more