› 1py0

X-ray diffraction
2Å resolution

Crystal structure of E51C/E54C Psaz from A.faecalis with CLaNP probe

Released:
DOI: 10.2210/pdb1py0/pdb
PDB entry 1py0 coloured by chain, front view.
PDB entry 1py0 coloured by chain, side view.
PDB entry 1py0 coloured by chain, top view.
Source organism: Alcaligenes faecalis
Primary publication:
A caged lanthanide complex as a paramagnetic shift agent for protein NMR.
Prudêncio M, Rohovec J, Peters JA, Tocheva E, Boulanger MJ, Murphy ME, Hupkes HJ, Kosters W, Impagliazzo A, Ubbink M
Chemistry 10 3252-60 (2004)
PMID: 15224334

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137608 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pseudoazurin Chain: A
Molecule details ›
Chain: A
Length: 125 amino acids
Theoretical weight: 13.49 KDa
Source organism: Alcaligenes faecalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04377 (Residues: 24-146; Coverage: 100%)
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand Y1 1 x Y1
Ligand SO4 1 x SO4
Ligand YMA 1 x YMA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P65
Unit cell:
a: 42.7Å b: 42.7Å c: 116.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.193 0.232
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

NMR spectroscopy of paramagnetic metalloproteins.
Bertini et al. (2005)
6 more

2 mentions without citation

Expanding the utility of NMR restraints with paramagnetic compounds: background and practical aspects.
Koehler et al. (2011)
1 more