› 1pvc

X-ray diffraction
2.4Å resolution

REFINEMENT OF THE SABIN STRAIN OF TYPE 3 POLIOVIRUS AT 2.4 ANGSTROMS AND THE CRYSTAL STRUCTURES OF ITS VARIANTS AT 2.9 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb1pvc/pdb
PDB entry 1pvc coloured by chain, front view.
PDB entry 1pvc coloured by chain, side view.
PDB entry 1pvc coloured by chain, top view.
Source organism: Poliovirus type 3 (strains P3/LEON/37 AND P3/LEON 12A[1]B)
Primary publication:
Refinement of the Sabin Strain of Type 3 Poliovirus at 2.4 Angstroms and the Crystal Structures of its Variants at 2.9 Angstroms Resolution
Syed R, Filman DJ, Hogle JM
To be published

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero 300-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136727 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
POLIOVIRUS TYPE 3, SABIN STRAIN Chain: 0
Molecule details ›
Chain: 0
Length: 4 amino acids
Theoretical weight: 446 Da
Source organism: Poliovirus type 3 (strains P3/LEON/37 AND P3/LEON 12A[1]B)
Capsid protein VP1 Chain: 1
Molecule details ›
Chain: 1
Length: 301 amino acids
Theoretical weight: 33.65 KDa
Source organism: Poliovirus type 3 (strains P3/LEON/37 AND P3/LEON 12A[1]B)
UniProt:
  • Canonical: P03302 (Residues: 578-878; Coverage: 14%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP2 Chain: 2
Molecule details ›
Chain: 2
Length: 271 amino acids
Theoretical weight: 30.17 KDa
Source organism: Poliovirus type 3 (strains P3/LEON/37 AND P3/LEON 12A[1]B)
UniProt:
  • Canonical: P03302 (Residues: 70-340; Coverage: 12%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP3 Chain: 3
Molecule details ›
Chain: 3
Length: 238 amino acids
Theoretical weight: 26.29 KDa
Source organism: Poliovirus type 3 (strains P3/LEON/37 AND P3/LEON 12A[1]B)
UniProt:
  • Canonical: P03302 (Residues: 341-578; Coverage: 11%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP4 Chain: 4
Molecule details ›
Chain: 4
Length: 68 amino acids
Theoretical weight: 7.32 KDa
Source organism: Poliovirus type 3 (strains P3/LEON/37 AND P3/LEON 12A[1]B)
UniProt:
  • Canonical: P03302 (Residues: 2-69; Coverage: 3%)
Sequence domains: Picornavirus coat protein (VP4)
Structure domains: Picornavirus coat protein VP4

Ligands and Environments

2 bound ligands:
Ligand SPH 1 x SPH
Ligand MYR 1 x MYR
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I222
Unit cell:
a: 321.06Å b: 358.62Å c: 381.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 not available not available

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