› 1pme

X-ray diffraction
2Å resolution

STRUCTURE OF PENTA MUTANT HUMAN ERK2 MAP KINASE COMPLEXED WITH A SPECIFIC INHIBITOR OF HUMAN P38 MAP KINASE

Released:
DOI: 10.2210/pdb1pme/pdb
PDB entry 1pme coloured by chain, front view.
PDB entry 1pme coloured by chain, side view.
PDB entry 1pme coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A single amino acid substitution makes ERK2 susceptible to pyridinyl imidazole inhibitors of p38 MAP kinase.
Fox T, Coll JT, Xie X, Ford PJ, Germann UA, Porter MD, Pazhanisamy S, Fleming MA, Galullo V, Su MS, Wilson KP
Protein Sci 7 2249-55 (1998)
PMID: 9827991

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151230 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 43.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P28482 (Residues: 1-360; Coverage: 100%)
Gene names: ERK2, MAPK1, PRKM1, PRKM2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand SB2 1 x SB2
1 modified residue:
Ligand CME 1 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 48.64Å b: 69.68Å c: 60.28Å
α: 90° β: 109.25° γ: 90°
R-values:
R R work R free
0.212 0.212 0.273
Expression system: Escherichia coli

Quick links

Citations

10 review citations

p38 MAP kinases: key signalling molecules as therapeutic targets for inflammatory diseases.
Kumar et al. (2003)
9 more

26 mentions without citation

Clinical Evidence and Potential Mechanisms in Treating Radiation Enteritis with Modified Baitouweng Decoction.
Wu et al. (2023)
25 more