› 1pjz

Solution NMR

Solution structure of thiopurine methyltransferase from Pseudomonas syringae

Released:
DOI: 10.2210/pdb1pjz/pdb
PDB entry 1pjz coloured by chain, ensemble of 20 models, front view.
PDB entry 1pjz coloured by chain, ensemble of 20 models, side view.
PDB entry 1pjz coloured by chain, ensemble of 20 models, top view.
Source organism: Pseudomonas syringae pv. pisi
Primary publication:
Tertiary structure of thiopurine methyltransferase from Pseudomonas syringae, a bacterial orthologue of a polymorphic, drug-metabolizing enzyme.
Scheuermann TH, Lolis E, Hodsdon ME
J Mol Biol 333 573-85 (2003)
PMID: 14556746

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131463 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiopurine S-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 203 amino acids
Theoretical weight: 22.59 KDa
Source organism: Pseudomonas syringae pv. pisi
Expression system: Escherichia coli
UniProt:
  • Canonical: O86262 (Residues: 18-218; Coverage: 92%)
Gene name: tpm
Sequence domains: Thiopurine S-methyltransferase (TPMT)
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing, molecular dynamics
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Microbiota and Drug Response in Inflammatory Bowel Disease.
Franzin et al. (2021)
2 more

3 mentions without citation

Structural basis of allele variation of human thiopurine-S-methyltransferase.
Wu et al. (2007)
2 more