› 1pd2

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF HEMATOPOIETIC PROSTAGLANDIN D SYNTHASE COMPLEX WITH GLUTATHIONE

Released:
DOI: 10.2210/pdb1pd2/pdb
PDB entry 1pd2 coloured by chain, front view.
PDB entry 1pd2 coloured by chain, side view.
PDB entry 1pd2 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Cloning and crystal structure of hematopoietic prostaglandin D synthase.
Kanaoka Y, Ago H, Inagaki E, Nanayama T, Miyano M, Kikuno R, Fujii Y, Eguchi N, Toh H, Urade Y, Hayaishi O
Cell 90 1085-95 (1997)
PMID: 9323136

Function and Biology Details

Reactions catalysed: 
RX + glutathione = HX + R-S-glutathione
(5Z,13E,15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E,15S)-9-alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128695 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hematopoietic prostaglandin D synthase Chains: 1, 2
Molecule details ›
Chains: 1, 2
Length: 199 amino acids
Theoretical weight: 23.32 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O35543 (Residues: 1-199; Coverage: 100%)
Gene names: Gsts, Hpgds, Pgds, Ptgds2
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GSH 2 x GSH
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P3121
Unit cell:
a: 56.5Å b: 56.5Å c: 233.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.204 0.289
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

30 review citations

Glutathione transferases.
Hayes et al. (2005)
29 more

6 mentions without citation

Enzymes of the cyclooxygenase pathways of prostanoid biosynthesis.
Smith et al. (2011)
5 more