› 1p5v

X-ray diffraction
1.7Å resolution

X-ray structure of the Caf1M:Caf1 chaperone:subunit preassembly complex

Released:
DOI: 10.2210/pdb1p5v/pdb
PDB entry 1p5v coloured by chain, front view.
PDB entry 1p5v coloured by chain, side view.
PDB entry 1p5v coloured by chain, top view.
Source organism: Yersinia pestis
Primary publication:
Structure and biogenesis of the capsular F1 antigen from Yersinia pestis: preserved folding energy drives fiber formation.
Zavialov AV, Berglund J, Pudney AF, Fooks LJ, Ibrahim TM, MacIntyre S, Knight SD
Cell 113 587-96 (2003)
PMID: 12787500

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150879 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chaperone protein caf1M Chain: A
Molecule details ›
Chain: A
Length: 235 amino acids
Theoretical weight: 26.33 KDa
Source organism: Yersinia pestis
Expression system: Escherichia coli
UniProt:
  • Canonical: P26926 (Residues: 24-258; Coverage: 99%)
Gene names: Y1098, YPMT1.82, YP_pMT084, caf1M
Sequence domains:
Structure domains: Immunoglobulins
F1 capsule antigen Chain: B
Molecule details ›
Chain: B
Length: 147 amino acids
Theoretical weight: 15.67 KDa
Source organism: Yersinia pestis
Expression system: Escherichia coli
UniProt:
  • Canonical: P26948 (Residues: 35-170; Coverage: 91%)
Gene names: Y1100, YPMT1.84, YP_pMT082, caf1
Sequence domains: Caf1 Capsule antigen
Structure domains: Fimbrial-type adhesion domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2, ESRF BEAMLINE ID14-4, ESRF BEAMLINE ID29, MAX II BEAMLINE I711
Spacegroup: P21
Unit cell:
a: 36Å b: 69.23Å c: 69.11Å
α: 90° β: 92.96° γ: 90°
R-values:
R R work R free
0.201 0.198 0.244
Expression system: Escherichia coli

Quick links

Citations

35 review citations

Evolution of the chaperone/usher assembly pathway: fimbrial classification goes Greek.
Nuccio et al. (2007)
34 more

7 mentions without citation

Insights into the Molecular Mechanisms of Alzheimer's and Parkinson's Diseases with Molecular Simulations: Understanding the Roles of Artificial and Pathological Missense Mutations in Intrinsically Disordered Proteins Related to Pathology.
Coskuner-Weber et al. (2018)
6 more