› 1p0k

X-ray diffraction
1.9Å resolution

IPP:DMAPP isomerase type II apo structure

Released:
DOI: 10.2210/pdb1p0k/pdb
PDB entry 1p0k coloured by chain, front view.
PDB entry 1p0k coloured by chain, side view.
PDB entry 1p0k coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structure of the type II isopentenyl diphosphate:dimethylallyl diphosphate isomerase from Bacillus subtilis.
Steinbacher S, Kaiser J, Gerhardt S, Eisenreich W, Huber R, Bacher A, Rohdich F
J Mol Biol 329 973-82 (2003)
PMID: 12798687

Function and Biology Details

Reaction catalysed: 
Isopentenyl diphosphate = prenyl diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo octamer
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156257 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isopentenyl-diphosphate delta-isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 349 amino acids
Theoretical weight: 37.26 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P50740 (Residues: 1-349; Coverage: 100%)
Gene names: BSU22870, fni, idi, ypgA
Sequence domains: FMN-dependent dehydrogenase
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I4
Unit cell:
a: 115.833Å b: 115.833Å c: 141.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.199 0.221
Expression system: Escherichia coli

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Citations

7 review citations

Mechanistic aspects of carotenoid biosynthesis.
Moise et al. (2014)
6 more

4 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
3 more