› 1ovd

X-ray diffraction
2.25Å resolution

THE K136E MUTANT OF LACTOCOCCUS LACTIS DIHYDROOROTATE DEHYDROGENASE A IN COMPLEX WITH OROTATE

Released:
DOI: 10.2210/pdb1ovd/pdb
PDB entry 1ovd coloured by chain, front view.
PDB entry 1ovd coloured by chain, side view.
PDB entry 1ovd coloured by chain, top view.
Source organism: Lactococcus lactis
Primary publication:
Lactococcus lactis dihydroorotate dehydrogenase A mutants reveal important facets of the enzymatic function.
Nørager S, Arent S, Björnberg O, Ottosen M, Lo Leggio L, Jensen KF, Larsen S
J Biol Chem 278 28812-22 (2003)
PMID: 12732650

Function and Biology Details

Reaction catalysed: 
(S)-dihydroorotate + fumarate = orotate + succinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106948 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydroorotate dehydrogenase A (fumarate) Chains: A, B
Molecule details ›
Chains: A, B
Length: 311 amino acids
Theoretical weight: 34.24 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: A2RJT9 (Residues: 1-311; Coverage: 100%)
Gene names: llmg_0952, pyrDA
Sequence domains: Dihydroorotate dehydrogenase
Structure domains:

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
3 bound ligands:
Ligand MG 2 x MG
Ligand ORO 2 x ORO
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P21
Unit cell:
a: 52.87Å b: 108.29Å c: 65.94Å
α: 90° β: 103.85° γ: 90°
R-values:
R R work R free
0.178 0.178 0.202
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Nucleotide metabolism and its control in lactic acid bacteria.
Kilstrup et al. (2005)
12 other articles

1 mention without citation

Expression, purification and crystallization of Trypanosoma cruzi dihydroorotate dehydrogenase complexed with orotate.
Inaoka et al. (2005)