› 1ou9

X-ray diffraction
1.8Å resolution

Structure of SspB, a AAA+ protease delivery protein

Released:
DOI: 10.2210/pdb1ou9/pdb
PDB entry 1ou9 coloured by chain, front view.
PDB entry 1ou9 coloured by chain, side view.
PDB entry 1ou9 coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
Structure of a delivery protein for an AAA+ protease in complex with a peptide degradation tag.
Levchenko I, Grant RA, Wah DA, Sauer RT, Baker TA
Mol Cell 12 365-72 (2003)
PMID: 14536076

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer
homo hexamer
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155262 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Stringent starvation protein B homolog Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 129 amino acids
Theoretical weight: 14.8 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P45206 (Residues: 1-129; Coverage: 86%)
Gene names: HI_1440, sspB
Sequence domains: Stringent starvation protein B
Structure domains: SspB-like

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2221
Unit cell:
a: 65.292Å b: 137.839Å c: 93.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.217 0.253
Expression system: Escherichia coli

Quick links

Citations

20 review citations

AAA+ proteases: ATP-fueled machines of protein destruction.
Sauer et al. (2011)
19 more

4 mentions without citation

Specificity versus stability in computational protein design.
Bolon et al. (2005)
3 more