› 1ota

X-ray diffraction
1.1Å resolution

E46Q MUTANT OF PHOTOACTIVE YELLOW PROTEIN, P63 AT 295K

Released:
DOI: 10.2210/pdb1ota/pdb
PDB entry 1ota coloured by chain, front view.
PDB entry 1ota coloured by chain, side view.
PDB entry 1ota coloured by chain, top view.
Source organism: Halorhodospira halophila
Primary publication:
Short hydrogen bonds in photoactive yellow protein.
Anderson S, Crosson S, Moffat K
Acta Crystallogr D Biol Crystallogr 60 1008-16 (2004)
PMID: 15159559

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147624 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Photoactive yellow protein Chain: A
Molecule details ›
Chain: A
Length: 125 amino acids
Theoretical weight: 13.89 KDa
Source organism: Halorhodospira halophila
Expression system: Escherichia coli
UniProt:
  • Canonical: P16113 (Residues: 1-125; Coverage: 100%)
Gene name: pyp
Sequence domains: PAS fold
Structure domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand HC4 1 x HC4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P63
Unit cell:
a: 66.917Å b: 66.917Å c: 41.002Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.153 0.151 0.18
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Ligand-binding PAS domains in a genomic, cellular, and structural context.
Henry et al. (2011)
8 more

3 mentions without citation

Combined probes of X-ray scattering and optical spectroscopy reveal how global conformational change is temporally and spatially linked to local structural perturbation in photoactive yellow protein.
Kim et al. (2016)
2 more