› 1ork

X-ray diffraction
2.4Å resolution

TET REPRESSOR, CLASS D IN COMPLEX WITH 9-(N,N-DIMETHYLGLYCYLAMIDO)-6-DEMETHYL-6-DEOXY-TETRACYCLINE

Released:
DOI: 10.2210/pdb1ork/pdb
PDB entry 1ork coloured by chain, front view.
PDB entry 1ork coloured by chain, side view.
PDB entry 1ork coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of the tet repressor in complex with a novel tetracycline, 9-(N,N-dimethylglycylamido)- 6-demethyl-6-deoxy-tetracycline.
Orth P, Schnappinger D, Sum PE, Ellestad GA, Hillen W, Saenger W, Hinrichs W
J Mol Biol 285 455-61 (1999)
PMID: 9878420

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142298 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetracycline repressor protein class D Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 23.29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACT4 (Residues: 2-208; Coverage: 95%)
Gene name: tetR
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ATC 1 x ATC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: I4122
Unit cell:
a: 68.94Å b: 68.94Å c: 180.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.275
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Regulation of bacterial drug export systems.
Grkovic et al. (2002)
4 more

1 mention without citation

The TetR family of transcriptional repressors.
Ramos et al. (2005)