› 1om2

Solution NMR

SOLUTION NMR STRUCTURE OF THE MITOCHONDRIAL PROTEIN IMPORT RECEPTOR TOM20 FROM RAT IN A COMPLEX WITH A PRESEQUENCE PEPTIDE DERIVED FROM RAT ALDEHYDE DEHYDROGENASE (ALDH)

Released:
DOI: 10.2210/pdb1om2/pdb
PDB entry 1om2 coloured by chain, ensemble of 20 models, front view.
PDB entry 1om2 coloured by chain, ensemble of 20 models, side view.
PDB entry 1om2 coloured by chain, ensemble of 20 models, top view.
Source organism: Rattus norvegicus
Primary publication:
Structural basis of presequence recognition by the mitochondrial protein import receptor Tom20.
Abe Y, Shodai T, Muto T, Mihara K, Torii H, Nishikawa S, Endo T, Kohda D
Cell 100 551-60 (2000)
PMID: 10721992

Function and Biology Details

Reaction catalysed: 
An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145970 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitochondrial import receptor subunit TOM20 homolog Chain: A
Molecule details ›
Chain: A
Length: 95 amino acids
Theoretical weight: 10.46 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q62760 (Residues: 51-145; Coverage: 66%)
Gene name: Tomm20
Sequence domains: MAS20 protein import receptor
Structure domains: Mitochondrial outer membrane translocase complex, subunit Tom20 domain
Aldehyde dehydrogenase, mitochondrial Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.23 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: P11884 (Residues: 12-22; Coverage: 2%)
Gene name: Aldh2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: TORSION ANGLE DYNAMICS FOLLOWED BY RESTRAINED ENERGY MINIMIZATION WITH AMBER FORCE FIELD
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

103 review citations

Translocation of proteins into mitochondria.
Neupert et al. (2007)
102 more

13 mentions without citation

The similarity between N-terminal targeting signals for protein import into different organelles and its evolutionary relevance.
Kunze et al. (2015)
12 more