› 1okg

X-ray diffraction
2.1Å resolution

3-mercaptopyruvate sulfurtransferase from Leishmania major

Released:
DOI: 10.2210/pdb1okg/pdb
PDB entry 1okg coloured by chain, front view.
PDB entry 1okg coloured by chain, side view.
PDB entry 1okg coloured by chain, top view.
Source organism: Leishmania major
Primary publication:
The crystal structure of Leishmania major 3-mercaptopyruvate sulfurtransferase. A three-domain architecture with a serine protease-like triad at the active site.
Alphey MS, Williams RA, Mottram JC, Coombs GH, Hunter WN
J Biol Chem 278 48219-27 (2003)
PMID: 12952945

Function and Biology Details

Reaction catalysed: 
(1a) 2-oxo-3-sulfanylpropanoate + [3-mercaptopyruvate sulfurtransferase]-L-cysteine = pyruvate + [3-mercaptopyruvate sulfurtransferase]-S-sulfanyl-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181583 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rhodanese domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 373 amino acids
Theoretical weight: 41.3 KDa
Source organism: Leishmania major
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7K9G0 (Residues: 1-370; Coverage: 100%)
Gene names: LMJF_05_0970, MST
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand SO3 1 x SO3
3 modified residues:
Ligand MSE 13 x MSE
Ligand CSR 1 x CSR
Ligand CSS 2 x CSS

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P42212
Unit cell:
a: 109.574Å b: 109.574Å c: 67.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.208 0.287
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Peptidyl-prolyl cis-trans isomerases (immunophilins) and their roles in parasite biochemistry, host-parasite interaction and antiparasitic drug action.
Bell et al. (2006)
6 more

3 mentions without citation

Identification of recurring protein structure microenvironments and discovery of novel functional sites around CYS residues.
Wu et al. (2010)
2 more