› 1oh9

X-ray diffraction
1.91Å resolution

Acetylglutamate kinase from Escherichia coli complexed with MgADP, N-acetyl-L-glutamate and the transition-state mimic AlF4-

Released:
DOI: 10.2210/pdb1oh9/pdb
PDB entry 1oh9 coloured by chain, front view.
PDB entry 1oh9 coloured by chain, side view.
PDB entry 1oh9 coloured by chain, top view.
Source organism: Escherichia coli BL21(DE3)
Primary publication:
The course of phosphorus in the reaction of N-acetyl-L-glutamate kinase, determined from the structures of crystalline complexes, including a complex with an AlF(4)(-) transition state mimic.
Gil-Ortiz F, Ramón-Maiques S, Fita I, Rubio V
J Mol Biol 331 231-44 (2003)
PMID: 12875848

Function and Biology Details

Reaction catalysed: 
ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141295 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetylglutamate kinase Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 27.19 KDa
Source organism: Escherichia coli BL21(DE3)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6C8 (Residues: 1-258; Coverage: 100%)
Gene names: JW5553, argB, b3959
Sequence domains: Amino acid kinase family
Structure domains: Acetylglutamate kinase-like

Ligands and Environments

4 bound ligands:
Ligand ADP 1 x ADP
Ligand NLG 1 x NLG
Ligand ALF 1 x ALF
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: C2221
Unit cell:
a: 59.457Å b: 72.22Å c: 107.214Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.186 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Arginine and nitrogen storage.
Llácer et al. (2008)
2 more

2 mentions without citation

On the conservation of the slow conformational dynamics within the amino acid kinase family: NAGK the paradigm.
Marcos et al. (2010)
1 more