› 1ob9

X-ray diffraction
2Å resolution

Holliday Junction Resolving Enzyme

Released:
DOI: 10.2210/pdb1ob9/pdb
PDB entry 1ob9 coloured by chain, front view.
PDB entry 1ob9 coloured by chain, side view.
PDB entry 1ob9 coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Substrate recognition and catalysis by the Holliday junction resolving enzyme Hje.
Middleton CL, Parker JL, Richard DJ, White MF, Bond CS
Nucleic Acids Res 32 5442-51 (2004)
PMID: 15479781

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189055 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Crossover junction endodeoxyribonuclease Hje Chain: A
Molecule details ›
Chain: A
Length: 135 amino acids
Theoretical weight: 15.48 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q97YX6 (Residues: 1-135; Coverage: 100%)
Gene names: SSO1176, hje
Sequence domains: Archaeal holliday junction resolvase (hjc)
Structure domains: Trna Endonuclease; Chain: A, domain 1

Ligands and Environments

2 bound ligands:
Ligand EDO 1 x EDO
Ligand FMT 7 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I41
Unit cell:
a: 76.57Å b: 76.57Å c: 88.944Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Cruciform structures are a common DNA feature important for regulating biological processes.
Brázda et al. (2011)
5 more

1 mention without citation

Crystal structure and initial characterization of a novel archaeal-like Holliday junction-resolving enzyme from Thermus thermophilus phage Tth15-6.
Ahlqvist et al. (2022)