› 1nz6

X-ray diffraction
2.5Å resolution

Crystal Structure of Auxilin J-Domain

Released:
DOI: 10.2210/pdb1nz6/pdb
PDB entry 1nz6 coloured by chain, front view.
PDB entry 1nz6 coloured by chain, side view.
PDB entry 1nz6 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structure-function analysis of the auxilin J-domain reveals an extended Hsc70 interaction interface.
Jiang J, Taylor AB, Prasad K, Ishikawa-Brush Y, Hart PJ, Lafer EM, Sousa R
Biochemistry 42 5748-53 (2003)
PMID: 12741832

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173296 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Auxilin Chains: A, B
Molecule details ›
Chains: A, B
Length: 101 amino acids
Theoretical weight: 12.02 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q27974 (Residues: 810-910; Coverage: 11%)
Gene name: DNAJC6
Structure domains: DnaJ domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: C2221
Unit cell:
a: 94.066Å b: 103.138Å c: 75.657Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.268
Expression system: Escherichia coli

Quick links

Citations

14 review citations

The J-protein family: modulating protein assembly, disassembly and translocation.
Walsh et al. (2004)
13 more

2 mentions without citation

Dynamical Structures of Hsp70 and Hsp70-Hsp40 Complexes.
Alderson et al. (2016)
1 more