› 1nxj

X-ray diffraction
1.9Å resolution

Structure of Rv3853 from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb1nxj/pdb
PDB entry 1nxj coloured by chain, front view.
PDB entry 1nxj coloured by chain, side view.
PDB entry 1nxj coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structure of a putative methyltransferase from Mycobacterium tuberculosis: misannotation of a genome clarified by protein structural analysis.
Johnston JM, Arcus VL, Morton CJ, Parker MW, Baker EN
J Bacteriol 185 4057-65 (2003)
PMID: 12837779

Function and Biology Details

Reactions catalysed: 
Oxaloacetate = pyruvate + CO(2)
2-hydroxy-4-oxobutane-1,2,4-tricarboxylate = oxaloacetate + pyruvate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161528 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 183 amino acids
Theoretical weight: 19.38 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGY3 (Residues: 2-157; Coverage: 99%)
Gene names: MTCY01A6.15c, Rv3853, rraA
Sequence domains: Aldolase/RraA
Structure domains: Ribonuclease E inhibitor RraA/RraA-like

Ligands and Environments

2 bound ligands:
Ligand GLV 3 x GLV
Ligand TLA 3 x TLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P63
Unit cell:
a: 102.462Å b: 102.462Å c: 117.494Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.19 0.215
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structural proteomics: a tool for genome annotation.
Yakunin et al. (2004)
3 more

4 mentions without citation

Crystal structure of a putative methyltransferase from Mycobacterium tuberculosis: misannotation of a genome clarified by protein structural analysis.
Johnston et al. (2003)
3 more