› 1nwc

X-ray diffraction
2.04Å resolution

Crystal Structure of Aspartate-Semialdehyde Dehydrogenase from Haemophilus influenzae

Released:
DOI: 10.2210/pdb1nwc/pdb
PDB entry 1nwc coloured by chain, front view.
PDB entry 1nwc coloured by chain, side view.
PDB entry 1nwc coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
Capture of an intermediate in the catalytic cycle of L-aspartate-beta-semialdehyde dehydrogenase.
Blanco J, Moore RA, Viola RE
Proc Natl Acad Sci U S A 100 12613-7 (2003)
PMID: 14559965

Function and Biology Details

Reaction catalysed: 
L-aspartate 4-semialdehyde + phosphate + NADP(+) = L-4-aspartyl phosphate + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155210 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartate-semialdehyde dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 371 amino acids
Theoretical weight: 40.58 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P44801 (Residues: 1-371; Coverage: 100%)
Gene names: HI_0646, asd
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P212121
Unit cell:
a: 53.764Å b: 109.878Å c: 113.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.257
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Inhibition of lysine biosynthesis: an evolving antibiotic strategy.
Hutton et al. (2007)
24 other articles

3 mentions without citation

Capture of an intermediate in the catalytic cycle of L-aspartate-beta-semialdehyde dehydrogenase.
Blanco et al. (2003)
2 more