› 1ns5

X-ray diffraction
1.68Å resolution

X-RAY STRUCTURE OF YBEA FROM E.COLI. NORTHEAST STRUCTURAL GENOMICS RESEARCH CONSORTIUM (NESG) TARGET ER45

Released:
DOI: 10.2210/pdb1ns5/pdb
PDB entry 1ns5 coloured by chain, front view.
PDB entry 1ns5 coloured by chain, side view.
PDB entry 1ns5 coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Benach J, Shen J, Rost B, Xiao R, Acton T, Montelione G, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + pseudouridine(1915) in 23S rRNA = S-adenosyl-L-homocysteine + N(3)-methylpseudouridine(1915) in 23S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141704 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA large subunit methyltransferase H Chains: A, B
Molecule details ›
Chains: A, B
Length: 155 amino acids
Theoretical weight: 17.55 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8I8 (Residues: 1-155; Coverage: 100%)
Gene names: JW0631, b0636, rlmH, ybeA
Sequence domains: Predicted SPOUT methyltransferase
Structure domains: Alpha/beta knot

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 44.922Å b: 58.652Å c: 57.415Å
α: 90° β: 102.15° γ: 90°
R-values:
R R work R free
0.145 0.145 0.213
Expression system: Escherichia coli

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