› 1nnf

X-ray diffraction
1.1Å resolution

Crystal Structure Analysis of Haemophlius Influenzae Ferric-ion Binding Protein H9Q Mutant Form

Released:
DOI: 10.2210/pdb1nnf/pdb
PDB entry 1nnf coloured by chain, front view.
PDB entry 1nnf coloured by chain, side view.
PDB entry 1nnf coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
High resolution structure of an alternate form of the ferric ion binding protein from Haemophilus influenzae.
Shouldice SR, Dougan DR, Skene RJ, Tari LW, McRee DE, Yu RH, Schryvers AB
J Biol Chem 278 11513-9 (2003)
PMID: 12533539

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron-utilization periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 309 amino acids
Theoretical weight: 33.76 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P35755 (Residues: 24-332; Coverage: 100%)
Gene names: HI_0097, fbp, fbpA, hitA
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

2 bound ligands:
Ligand FE 1 x FE
Ligand EDT 1 x EDT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P21212
Unit cell:
a: 105.101Å b: 75.344Å c: 33.503Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.158 0.18
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Genomic insights into the iron uptake mechanisms of the biomining microorganism Acidithiobacillus ferrooxidans.
Quatrini et al. (2005)
21 other articles