› 1nkd

X-ray diffraction
1.09Å resolution

ATOMIC RESOLUTION (1.07 ANGSTROMS) STRUCTURE OF THE ROP MUTANT <2AA>

Released:
DOI: 10.2210/pdb1nkd/pdb
PDB entry 1nkd coloured by chain, front view.
PDB entry 1nkd coloured by chain, side view.
PDB entry 1nkd coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural parameters for proteins derived from the atomic resolution (1.09 A) structure of a designed variant of the ColE1 ROP protein.
Vlassi M, Dauter Z, Wilson KS, Kokkinidis M
Acta Crystallogr. D Biol. Crystallogr. 54 1245-60 (1998)
PMID: 10089502

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136490 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulatory protein rop Chain: A
Molecule details ›
Chain: A
Length: 65 amino acids
Theoretical weight: 7.38 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03051 (Residues: 1-29, 30-30, 31-63; Coverage: 100%)
Gene name: rop
Sequence domains: Rop protein
Structure domains: Helix Hairpins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: C2
Unit cell:
a: 47.06Å b: 37.88Å c: 31.65Å
α: 90° β: 100.8° γ: 90°
R-values:
R R work R free
0.101 not available 0.134
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Conformation-dependent backbone geometry restraints set a new standard for protein crystallographic refinement.
Moriarty et al. (2014)
1 more

12 mentions without citation

A graph-theory algorithm for rapid protein side-chain prediction.
Canutescu et al. (2003)
11 more