› 1ng9

X-ray diffraction
2.6Å resolution

E.coli MutS R697A: an ATPase-asymmetry mutant

Released:
DOI: 10.2210/pdb1ng9/pdb
PDB entry 1ng9 coloured by chain, front view.
PDB entry 1ng9 coloured by chain, side view.
PDB entry 1ng9 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The alternating ATPase domains of MutS control DNA mismatch repair.
Lamers MH, Winterwerp HH, Sixma TK
EMBO J 22 746-56 (2003)
PMID: 12554674

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114244 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA mismatch repair protein MutS Chains: A, B
Molecule details ›
Chains: A, B
Length: 800 amino acids
Theoretical weight: 89.52 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P23909 (Residues: 1-800; Coverage: 94%)
Gene names: JW2703, b2733, fdv, mutS
Sequence domains:
Structure domains:
5'-D(*AP*GP*CP*TP*GP*CP*CP*AP*GP*GP*CP*AP*CP*CP*AP*GP*TP*GP*TP*CP*AP*GP*CP*GP*TP*CP*CP*TP*AP*T)-3' Chain: E
Molecule details ›
Chain: E
Length: 30 nucleotides
Theoretical weight: 9.18 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*AP*TP*AP*GP*GP*AP*CP*GP*CP*TP*GP*AP*CP*AP*CP*TP*GP*GP*TP*GP*CP*TP*TP*GP*GP*CP*AP*GP*CP*T)-3' Chain: F
Molecule details ›
Chain: F
Length: 30 nucleotides
Theoretical weight: 9.28 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 89.902Å b: 92.404Å c: 261.593Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.215 0.249
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

21 review citations

DNA mismatch repair.
Kunkel et al. (2005)
20 more

4 mentions without citation

Structure and dynamics of adeno-associated virus serotype 1 VP1-unique N-terminal domain and its role in capsid trafficking.
Venkatakrishnan et al. (2013)
3 more