› 1n20

X-ray diffraction
2.3Å resolution

(+)-Bornyl Diphosphate Synthase: Complex with Mg and 3-aza-2,3-dihydrogeranyl diphosphate

Released:
DOI: 10.2210/pdb1n20/pdb
PDB entry 1n20 coloured by chain, front view.
PDB entry 1n20 coloured by chain, side view.
PDB entry 1n20 coloured by chain, top view.
Source organism: Salvia officinalis
Primary publication:
Bornyl diphosphate synthase: structure and strategy for carbocation manipulation by a terpenoid cyclase.
Whittington DA, Wise ML, Urbansky M, Coates RM, Croteau RB, Christianson DW
Proc Natl Acad Sci U S A 99 15375-80 (2002)
PMID: 12432096

Function and Biology Details

Reactions catalysed: 
Geranyl diphosphate = (+)-camphene + diphosphate
Geranyl diphosphate = (+)-alpha-pinene + diphosphate
Geranyl diphosphate = (+)-bornyl diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131339 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(+)-bornyl diphosphate synthase, chloroplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 549 amino acids
Theoretical weight: 64.04 KDa
Source organism: Salvia officinalis
Expression system: Escherichia coli
UniProt:
  • Canonical: O81192 (Residues: 50-598; Coverage: 92%)
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 6 x MG
Ligand 3AG 2 x 3AG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 101.08Å b: 117.54Å c: 120.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.228
Expression system: Escherichia coli

Quick links

Citations

26 review citations

The family of terpene synthases in plants: a mid-size family of genes for specialized metabolism that is highly diversified throughout the kingdom.
Chen et al. (2011)
25 more

15 mentions without citation

Terpenoid synthase structures: a so far incomplete view of complex catalysis.
Gao et al. (2012)
14 more