› 1mzf

X-ray diffraction
2.4Å resolution

Human Factor inhibiting HIF (FIH1) in Complex with 2-oxoglutarate

Released:
DOI: 10.2210/pdb1mzf/pdb
PDB entry 1mzf coloured by chain, front view.
PDB entry 1mzf coloured by chain, side view.
PDB entry 1mzf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of factor-inhibiting hypoxia-inducible factor 1: An asparaginyl hydroxylase involved in the hypoxic response pathway.
Dann CE, Bruick RK, Deisenhofer J
Proc Natl Acad Sci U S A 99 15351-6 (2002)
PMID: 12432100

Function and Biology Details

Reactions catalysed: 
Hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O(2) = hypoxia-inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO(2)
[Ankyrin-repeat domain protein]-L-asparagine + 2-oxoglutarate + O(2) = [ankyrin-repeat domain protein]-(3S)-3-hydroxy-L-asparagine + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192661 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxia-inducible factor 1-alpha inhibitor Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 40.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NWT6 (Residues: 1-349; Coverage: 100%)
Gene names: FIH1, HIF1AN
Sequence domains: Cupin-like domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FE2 1 x FE2
Ligand AKG 1 x AKG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P41212
Unit cell:
a: 86.46Å b: 86.46Å c: 147.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.232 0.244
Expression system: Escherichia coli

Quick links

Citations

37 review citations

Intrinsically unstructured proteins and their functions.
Dyson et al. (2005)
36 more

5 mentions without citation

Structural Basis of Prolyl Hydroxylase Domain Inhibition by Molidustat.
Figg et al. (2021)
4 more