› 1mxt

X-ray diffraction
0.95Å resolution

Atomic resolution structure of Cholesterol oxidase (Streptomyces sp. SA-COO)

Released:
DOI: 10.2210/pdb1mxt/pdb
PDB entry 1mxt coloured by chain, front view.
PDB entry 1mxt coloured by chain, side view.
PDB entry 1mxt coloured by chain, top view.
Source organism: Streptomyces sp.
Primary publication:
Sub-atomic resolution crystal structure of cholesterol oxidase: what atomic resolution crystallography reveals about enzyme mechanism and the role of the FAD cofactor in redox activity.
Lario PI, Sampson N, Vrielink A
J Mol Biol 326 1635-50 (2003)
PMID: 12595270

Function and Biology Details

Reactions catalysed: 
Cholesterol + O(2) = cholest-5-en-3-one + H(2)O(2)
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146297 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cholesterol oxidase Chain: A
Molecule details ›
Chain: A
Length: 504 amino acids
Theoretical weight: 54.97 KDa
Source organism: Streptomyces sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: P12676 (Residues: 43-546; Coverage: 100%)
Gene name: choA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAE 1 x FAE
2 bound ligands:
Ligand SO4 1 x SO4
Ligand OXY 1 x OXY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21
Unit cell:
a: 51.227Å b: 72.902Å c: 62.947Å
α: 90° β: 105.1° γ: 90°
R-values:
R R work R free
0.11 0.11 0.132
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Gates of enzymes.
Gora et al. (2013)
11 more

13 mentions without citation

Alteration of Electron Acceptor Preferences in the Oxidative Half-Reaction of Flavin-Dependent Oxidases and Dehydrogenases.
Hiraka et al. (2020)
12 more