1mx9

X-ray diffraction
2.9Å resolution

Crystal Structure of Human Liver Carboxylesterase in complexed with naloxone methiodide, a heroin analogue

Released:

Function and Biology Details

Reactions catalysed:
A carboxylic ester + H(2)O = an alcohol + a carboxylate
A steryl ester + H(2)O = a sterol + a fatty acid
4-methylumbelliferyl acetate + H(2)O = 4-methylumbelliferone + acetate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
PDBe Complex ID:
PDB-CPX-149883 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Liver carboxylesterase 1 Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 548 amino acids
Theoretical weight: 60.51 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P23141 (Residues: 19-567; Coverage: 100%)
Gene names: CES1, CES2, SES1
Sequence domains: Carboxylesterase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P1
Unit cell:
a: 91.174Å b: 120.711Å c: 177.017Å
α: 90.28° β: 89.32° γ: 99.22°
R-values:
R R work R free
0.247 0.214 0.28
Expression system: Spodoptera frugiperda