› 1mss

X-ray diffraction
2.4Å resolution

LARGE SCALE STRUCTURAL REARRANGEMENTS OF THE FRONT LOOPS IN MONOMERISED TRIOSEPHOSPHATE ISOMERASE, AS DEDUCED FROM THE COMPARISON OF THE STRUCTURAL PROPERTIES OF MONOTIM AND ITS POINT MUTATION VARIANT MONOSS

Released:
DOI: 10.2210/pdb1mss/pdb
PDB entry 1mss coloured by chain, front view.
PDB entry 1mss coloured by chain, side view.
PDB entry 1mss coloured by chain, top view.
Source organism: Trypanosoma brucei brucei
Primary publication:
Three new crystal structures of point mutation variants of monoTIM: conformational flexibility of loop-1, loop-4 and loop-8.
Borchert TV, Kishan KV, Zeelen JP, Schliebs W, Thanki N, Abagyan R, Jaenicke R, Wierenga RK
Structure 3 669-79 (1995)
PMID: 8591044

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate = glycerone phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138176 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Triosephosphate isomerase, glycosomal Chains: A, B
Molecule details ›
Chains: A, B
Length: 243 amino acids
Theoretical weight: 26.02 KDa
Source organism: Trypanosoma brucei brucei
Expression system: Not provided
UniProt:
  • Canonical: P04789 (Residues: 1-250; Coverage: 97%)
Sequence domains: Triosephosphate isomerase
Structure domains: Aldolase class I

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P1
Unit cell:
a: 46.57Å b: 46.58Å c: 66.65Å
α: 94.47° β: 69.82° γ: 75.83°
R-values:
R R work R free
0.198 0.198 not available
Expression system: Not provided

Quick links

Citations

1 review citation

Triosephosphate isomerase: a highly evolved biocatalyst.
Wierenga et al. (2010)
20 other articles

3 mentions without citation

Entamoeba histolytica modulates a complex repertoire of novel genes in response to oxidative and nitrosative stresses: implications for amebic pathogenesis.
Vicente et al. (2009)
2 more