› 1mn7

X-ray diffraction
2.15Å resolution

NDP kinase mutant (H122G;N119S;F64W) in complex with aBAZTTP

Released:
DOI: 10.2210/pdb1mn7/pdb
PDB entry 1mn7 coloured by chain, front view.
PDB entry 1mn7 coloured by chain, side view.
PDB entry 1mn7 coloured by chain, top view.
Source organism: Dictyostelium discoideum
Primary publication:
Improving nucleoside diphosphate kinase for antiviral nucleotide analogs activation.
Gallois-Montbrun S, Schneider B, Chen Y, Giacomoni-Fernandes V, Mulard L, Morera S, Janin J, Deville-Bonne D, Veron M
J Biol Chem 277 39953-9 (2002)
PMID: 12171931

Function and Biology Details

Reaction catalysed: 
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149819 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoside diphosphate kinase, cytosolic Chains: A, B
Molecule details ›
Chains: A, B
Length: 155 amino acids
Theoretical weight: 16.75 KDa
Source organism: Dictyostelium discoideum
Expression system: Escherichia coli
UniProt:
  • Canonical: P22887 (Residues: 1-155; Coverage: 100%)
Gene names: DDB_G0273069, DDB_G0273805, gip17, ndkB, ndkC-1, ndkC-2
Sequence domains: Nucleoside diphosphate kinase
Structure domains: Nucleoside diphosphate kinase-like domain

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ABT 2 x ABT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P63
Unit cell:
a: 71.238Å b: 71.238Å c: 106.677Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.232 0.295
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Update on human herpesvirus 6 biology, clinical features, and therapy.
De Bolle et al. (2005)
5 more