› 1ml7

X-ray diffraction
1.25Å resolution

Crystal structure of nitrophorin 4 complexed with 4-iodopyrazole

Released:
DOI: 10.2210/pdb1ml7/pdb
PDB entry 1ml7 coloured by chain, front view.
PDB entry 1ml7 coloured by chain, side view.
PDB entry 1ml7 coloured by chain, top view.
Source organism: Rhodnius prolixus
Primary publication:
Axial ligand complexes of the Rhodnius nitrophorins: reduction potentials, binding constants, EPR spectra, and structures of the 4-iodopyrazole and imidazole complexes of NP4.
Berry RE, Ding XD, Shokhireva TKh, Weichsel A, Montfort WR, Walker FA
J. Biol. Inorg. Chem. 9 135-44 (2004)
PMID: 14673714

Function and Biology Details

Reaction catalysed: 
3 nitrite + 2 H(+) = 2 nitric oxide + nitrate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188198 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrophorin-4 Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 20.29 KDa
Source organism: Rhodnius prolixus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q94734 (Residues: 22-205; Coverage: 100%)
Sequence domains: Nitrophorin
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:
Ligand HEV 1 x HEV
Ligand PYZ 2 x PYZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2
Unit cell:
a: 70.404Å b: 42.763Å c: 52.769Å
α: 90° β: 94.05° γ: 90°
R-values:
R R work R free
0.145 0.142 0.194
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Nitrophorins: nitrite disproportionation reaction and other novel functionalities of insect heme-based nitric oxide transport proteins.
Knipp et al. (2011)
1 more

3 mentions without citation

Comparative performance of several flexible docking programs and scoring functions: enrichment studies for a diverse set of pharmaceutically relevant targets.
Zhou et al. (2007)
2 more