› 1mec

X-ray diffraction
3.2Å resolution

CONFORMATIONAL VARIABILITY OF A PICORNAVIRUS CAPSID: PH-DEPENDENT STRUCTURAL CHANGES OF MENGO VIRUS RELATED TO ITS HOST RECEPTOR ATTACHMENT SITE AND DISASSEMBLY

Released:
DOI: 10.2210/pdb1mec/pdb
PDB entry 1mec coloured by chain, front view.
PDB entry 1mec coloured by chain, side view.
PDB entry 1mec coloured by chain, top view.
Source organism: Mengo virus
Primary publication:
Conformational variability of a picornavirus capsid: pH-dependent structural changes of Mengo virus related to its host receptor attachment site and disassembly.
Kim S, Boege U, Krishnaswamy S, Minor I, Smith TJ, Luo M, Scraba DG, Rossmann MG
Virology 175 176-90 (1990)
PMID: 2155508

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero 240-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146133 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Capsid protein VP1 Chain: 1
Molecule details ›
Chain: 1
Length: 274 amino acids
Theoretical weight: 30.27 KDa
Source organism: Mengo virus
Expression system: Not provided
UniProt:
  • Canonical: P12296 (Residues: 625-898; Coverage: 12%)
Structure domains: Jelly Rolls
Capsid protein VP2 Chain: 2
Molecule details ›
Chain: 2
Length: 256 amino acids
Theoretical weight: 28.79 KDa
Source organism: Mengo virus
Expression system: Not provided
UniProt:
  • Canonical: P12296 (Residues: 138-393; Coverage: 11%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP3 Chain: 3
Molecule details ›
Chain: 3
Length: 231 amino acids
Theoretical weight: 25.19 KDa
Source organism: Mengo virus
Expression system: Not provided
UniProt:
  • Canonical: P12296 (Residues: 394-624; Coverage: 10%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP4 Chain: 4
Molecule details ›
Chain: 4
Length: 70 amino acids
Theoretical weight: 7.34 KDa
Source organism: Mengo virus
Expression system: Not provided
UniProt:
  • Canonical: P12296 (Residues: 68-137; Coverage: 3%)
Sequence domains: Viral protein VP4 subunit
Structure domains: Capsid protein VP4 superfamily, Picornavirus

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 439.8Å b: 426.9Å c: 421.2Å
α: 90° β: 90° γ: 90°
Expression system: Not provided

Quick links

Citations

3 review citations

Picornavirus-receptor interactions.
Rossmann et al. (2002)
2 more

3 mentions without citation

A sensor-adaptor mechanism for enterovirus uncoating from structures of EV71.
Wang et al. (2012)
2 more