› 1mcw

X-ray diffraction
3.5Å resolution

THREE-DIMENSIONAL STRUCTURE OF A HYBRID LIGHT CHAIN DIMER. PROTEIN ENGINEERING OF A BINDING CAVITY

Released:
DOI: 10.2210/pdb1mcw/pdb
PDB entry 1mcw coloured by chain, front view.
PDB entry 1mcw coloured by chain, side view.
PDB entry 1mcw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Three-dimensional structure of a hybrid light chain dimer: protein engineering of a binding cavity.
Ely KR, Herron JN, Edmundson AB
Mol. Immunol. 27 101-14 (1990)
PMID: 2108322

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208468 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
IMMUNOGLOBULIN WEIR (LIGHT CHAIN) Chain: W
Molecule details ›
Chain: W
Length: 216 amino acids
Theoretical weight: 22.91 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins
Immunoglobulin lambda variable 2-8 Chain: M
Molecule details ›
Chain: M
Length: 216 amino acids
Theoretical weight: 22.83 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01709 (Residues: 20-118; Coverage: 100%)
Gene name: IGLV2-8
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3121
Unit cell:
a: 72.3Å b: 72.3Å c: 185.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 not available not available

Quick links

Citations

3 review citations

The surrogate light chain in B-cell development.
Melchers et al. (1993)
2 more

3 mentions without citation

Automated minimization of steric clashes in protein structures.
Ramachandran et al. (2011)
2 more