› 1m0d

X-ray diffraction
1.9Å resolution

Crystal Structure of Bacteriophage T7 Endonuclease I with a Wild-Type Active Site and Bound Manganese Ions

Released:
DOI: 10.2210/pdb1m0d/pdb
PDB entry 1m0d coloured by chain, front view.
PDB entry 1m0d coloured by chain, side view.
PDB entry 1m0d coloured by chain, top view.
Source organism: Escherichia phage T7
Primary publication:
Metal ions bound at the active site of the junction-resolving enzyme T7 endonuclease I.
Hadden JM, Déclais AC, Phillips SE, Lilley DM
EMBO J 21 3505-15 (2002)
PMID: 12093751

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132788 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endonuclease I Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 138 amino acids
Theoretical weight: 16.07 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00641 (Residues: 12-149; Coverage: 93%)
Gene name: 3
Sequence domains: Phage endonuclease I
Structure domains: Restriction Endonuclease

Ligands and Environments

2 bound ligands:
Ligand MN 8 x MN
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21212
Unit cell:
a: 123.81Å b: 133.96Å c: 61.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.234
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

New insight into the recognition of branched DNA structure by junction-resolving enzymes.
Déclais et al. (2008)
5 more

9 mentions without citation

Holliday junction resolvases.
Wyatt et al. (2014)
8 more