› 1lxg

Solution NMR

Solution structure of alpha-cobratoxin complexed with a cognate peptide (structure ensemble)

Released:
DOI: 10.2210/pdb1lxg/pdb
PDB entry 1lxg coloured by chain, ensemble of 10 models, front view.
PDB entry 1lxg coloured by chain, ensemble of 10 models, side view.
PDB entry 1lxg coloured by chain, ensemble of 10 models, top view.
Source organisms:
Primary publication:
NMR-based binding screen and structural analysis of the complex formed between alpha-cobratoxin and an 18-mer cognate peptide derived from the alpha 1 subunit of the nicotinic acetylcholine receptor from Torpedo californica.
Zeng H, Hawrot E
J Biol Chem 277 37439-45 (2002)
PMID: 12133834

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134298 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Alpha-cobratoxin Chain: A
Molecule details ›
Chain: A
Length: 71 amino acids
Theoretical weight: 7.84 KDa
Source organism: Naja kaouthia
UniProt:
  • Canonical: P01391 (Residues: 1-71; Coverage: 100%)
Structure domains: CD59
Acetylcholine receptor subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 19 amino acids
Theoretical weight: 2.42 KDa
Source organism: Tetronarce californica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02710 (Residues: 205-222; Coverage: 4%)
Gene name: CHRNA1

Ligands and Environments

No bound ligands
1 modified residue:
Ligand HSL 1 x HSL

Experiments and Validation Details

Entry percentile scores
Refinement method: distance geometry and simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Snake and snail toxins acting on nicotinic acetylcholine receptors: fundamental aspects and medical applications.
Tsetlin et al. (2004)
4 more

1 mention without citation

Virtual screening against alpha-cobratoxin.
Utsintong et al. (2009)