› 1l8t

X-ray diffraction
2.4Å resolution

Crystal Structure Of 3',5"-Aminoglycoside Phosphotransferase Type IIIa ADP Kanamycin A Complex

Released:
DOI: 10.2210/pdb1l8t/pdb
PDB entry 1l8t coloured by chain, front view.
PDB entry 1l8t coloured by chain, side view.
PDB entry 1l8t coloured by chain, top view.
Source organism: Enterococcus faecalis
Primary publication:
Substrate promiscuity of an aminoglycoside antibiotic resistance enzyme via target mimicry.
Fong DH, Berghuis AM
EMBO J 21 2323-31 (2002)
PMID: 12006485

Function and Biology Details

Reaction catalysed: 
ATP + kanamycin = ADP + kanamycin 3'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141232 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside 3'-phosphotransferase Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 30.88 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A3Y5 (Residues: 2-264; Coverage: 100%)
Gene name: aphA
Sequence domains: Phosphotransferase enzyme family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand ADP 1 x ADP
Ligand KAN 1 x KAN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P4322
Unit cell:
a: 46.629Å b: 46.629Å c: 301.289Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.291
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Molecular recognition of aminoglycoside antibiotics by ribosomal RNA and resistance enzymes: an analysis of x-ray crystal structures.
Vicens et al. (2003)
14 more

20 mentions without citation

Aminoglycoside modifying enzymes.
Ramirez et al. (2010)
19 more