› 1k77

X-ray diffraction
1.63Å resolution

Crystal Structure of EC1530, a Putative Oxygenase from Escherichia coli

Released:
DOI: 10.2210/pdb1k77/pdb
PDB entry 1k77 coloured by chain, front view.
PDB entry 1k77 coloured by chain, side view.
PDB entry 1k77 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of Escherichia coli EC1530, a glyoxylate induced protein YgbM.
Kim Y, Skarina T, Beasley S, Laskowski R, Arrowsmith C, Joachimiak A, Edwards A, Savchenko A
Proteins 48 427-30 (2002)
PMID: 12112708

Function and Biology Details

Reaction catalysed: 
2-dehydro-D-erythronate = 3-dehydro-D-erythronate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175155 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-oxo-tetronate isomerase Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 29.58 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46891 (Residues: 1-258; Coverage: 100%)
Gene names: JW2709, b2739, otnI, ygbM
Sequence domains: Xylose isomerase-like TIM barrel
Structure domains: Divalent-metal-dependent TIM barrel enzymes

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand GOL 2 x GOL
Ligand FMT 1 x FMT
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 104.907Å b: 74.368Å c: 39.376Å
α: 90° β: 98.81° γ: 90°
R-values:
R R work R free
0.196 0.194 0.214
Expression system: Escherichia coli

Quick links

Citations

3 mentions without citation

Annotation of proteins of unknown function: initial enzyme results.
McKay et al. (2015)
2 more