› 1k72

X-ray diffraction
1.8Å resolution

The X-ray Crystal Structure Of Cel9G Complexed With cellotriose

Released:
DOI: 10.2210/pdb1k72/pdb
PDB entry 1k72 coloured by chain, front view.
PDB entry 1k72 coloured by chain, side view.
PDB entry 1k72 coloured by chain, top view.
Source organism: Ruminiclostridium cellulolyticum
Primary publication:
X-Ray crystal structure of the multidomain endoglucanase Cel9G from Clostridium cellulolyticum complexed with natural and synthetic cello-oligosaccharides.
Mandelman D, Belaich A, Belaich JP, Aghajari N, Driguez H, Haser R
J Bacteriol 185 4127-35 (2003)
PMID: 12837787

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153470 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Endoglucanase G Chains: A, B
Molecule details ›
Chains: A, B
Length: 614 amino acids
Theoretical weight: 68.11 KDa
Source organism: Ruminiclostridium cellulolyticum
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P37700 (Residues: 36-649; Coverage: 89%)
Gene names: Ccel_0731, celCCG
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
4 bound ligands:
Ligand GLC 1 x GLC
Ligand CA 4 x CA
Ligand MG 4 x MG
Ligand GOL 2 x GOL
1 modified residue:
Ligand SCH 2 x SCH

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR571
Spacegroup: P1
Unit cell:
a: 57Å b: 57.6Å c: 86.8Å
α: 93.6° β: 100.8° γ: 99.5°
R-values:
R R work R free
0.166 0.166 0.198
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Clostridium cellulolyticum: model organism of mesophilic cellulolytic clostridia.
Desvaux M. (2005)
3 more

3 mentions without citation

X-Ray crystal structure of the multidomain endoglucanase Cel9G from Clostridium cellulolyticum complexed with natural and synthetic cello-oligosaccharides.
Mandelman et al. (2003)
2 more