› 1k2r

X-ray diffraction
2.15Å resolution

Structure of rat brain nNOS heme domain complexed with NG-nitro-L-arginine

Released:
DOI: 10.2210/pdb1k2r/pdb
PDB entry 1k2r coloured by chain, front view.
PDB entry 1k2r coloured by chain, side view.
PDB entry 1k2r coloured by chain, top view.
Source organism: Rattus norvegicus
Entry authors: Li H, Martasek P, Masters BSS, Poulos TL, Raman CS

Function and Biology Details

Reaction catalysed: 
(1a) 2 L-arginine + 2 NADPH + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 NADP(+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151520 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitric oxide synthase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 419 amino acids
Theoretical weight: 48.56 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P29476 (Residues: 299-717; Coverage: 29%)
Gene names: Bnos, Nos1
Sequence domains: Nitric oxide synthase, oxygenase domain
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
4 bound ligands:
Ligand ACT 2 x ACT
Ligand H4B 2 x H4B
Ligand NRG 2 x NRG
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 51.65Å b: 110.49Å c: 164.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.27
Expression system: Escherichia coli

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