› 1jvi

X-ray diffraction
2.2Å resolution

THE 2.2 ANGSTROM RESOLUTION STRUCTURE OF BACILLUS SUBTILIS LUXS/RIBOSILHOMOCYSTEINE COMPLEX

Released:
DOI: 10.2210/pdb1jvi/pdb
PDB entry 1jvi coloured by chain, front view.
PDB entry 1jvi coloured by chain, side view.
PDB entry 1jvi coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
The 1.2 A structure of a novel quorum-sensing protein, Bacillus subtilis LuxS.
Ruzheinikov SN, Das SK, Sedelnikova SE, Hartley A, Foster SJ, Horsburgh MJ, Cox AG, McCleod CW, Mekhalfia A, Blackburn GM, Rice DW, Baker PJ
J Mol Biol 313 111-22 (2001)
PMID: 11601850

Function and Biology Details

Reaction catalysed: 
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128577 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-ribosylhomocysteine lyase Chain: A
Molecule details ›
Chain: A
Length: 157 amino acids
Theoretical weight: 17.79 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O34667 (Residues: 1-157; Coverage: 100%)
Gene names: BSU30670, luxS, ytjB
Sequence domains: S-Ribosylhomocysteinase (LuxS)
Structure domains: S-ribosylhomocysteinase (LuxS)

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand SO4 1 x SO4
Ligand RHC 1 x RHC
Ligand HCS 1 x HCS
1 modified residue:
Ligand OCS 1 x OCS

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P6522
Unit cell:
a: 62.095Å b: 62.095Å c: 150.051Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.174 0.242
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Making 'sense' of metabolism: autoinducer-2, LuxS and pathogenic bacteria.
Vendeville et al. (2005)
11 more

3 mentions without citation

Cysteine Oxidation in Proteins: Structure, Biophysics, and Simulation.
Garrido Ruiz et al. (2022)
2 more