› 1jni

X-ray diffraction
1.25Å resolution

Structure of the NapB subunit of the periplasmic nitrate reductase from Haemophilus influenzae.

Released:
DOI: 10.2210/pdb1jni/pdb
PDB entry 1jni coloured by chain, front view.
PDB entry 1jni coloured by chain, side view.
PDB entry 1jni coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
The 1.25 A resolution structure of the diheme NapB subunit of soluble nitrate reductase reveals a novel cytochrome c fold with a stacked heme arrangement.
Brigé A, Leys D, Meyer TE, Cusanovich MA, Van Beeumen JJ
Biochemistry 41 4827-36 (2002)
PMID: 11939777

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155195 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic nitrate reductase, electron transfer subunit Chain: A
Molecule details ›
Chain: A
Length: 123 amino acids
Theoretical weight: 13.44 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P44654 (Residues: 28-150; Coverage: 99%)
Gene names: HI_0347, napB
Sequence domains: Nitrate reductase cytochrome c-type subunit (NapB)
Structure domains: Flavocytochrome C3; Chain A

Ligands and Environments

1 bound ligand:
Ligand HEC 2 x HEC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID13
Spacegroup: P4212
Unit cell:
a: 78.752Å b: 78.752Å c: 29.047Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.17 0.214
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Bacterial nitrate reductases: Molecular and biological aspects of nitrate reduction.
González et al. (2006)
5 more

6 mentions without citation

The mononuclear molybdenum enzymes.
Hille et al. (2014)
5 more