› 1jif

X-ray diffraction
1.6Å resolution

Crystal structure of bleomycin-binding protein from bleomycin-producing Streptomyces verticillus complexed with copper(II)-bleomycin

Released:
DOI: 10.2210/pdb1jif/pdb
PDB entry 1jif coloured by chain, front view.
PDB entry 1jif coloured by chain, side view.
PDB entry 1jif coloured by chain, top view.
Source organism: Streptomyces verticillus
Primary publication:
The 1.6-A crystal structure of the copper(II)-bound bleomycin complexed with the bleomycin-binding protein from bleomycin-producing Streptomyces verticillus.
Sugiyama M, Kumagai T, Hayashida M, Maruyama M, Matoba Y
J Biol Chem 277 2311-20 (2002)
PMID: 11706014

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176077 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bleomycin resistance protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 122 amino acids
Theoretical weight: 13.24 KDa
Source organism: Streptomyces verticillus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53793 (Residues: 1-122; Coverage: 100%)
Gene name: ble
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

3 bound ligands:
Ligand CU 2 x CU
Ligand CL 2 x CL
Ligand BLM 2 x BLM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 35.73Å b: 83.47Å c: 43.06Å
α: 90° β: 106.2° γ: 90°
R-values:
R R work R free
0.15 not available 0.207
Expression system: Escherichia coli

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Citations

6 review citations

Bleomycins: towards better therapeutics.
Chen et al. (2005)
5 more

4 mentions without citation

Characterization of BRPMBL, the Bleomycin Resistance Protein Associated with the Carbapenemase NDM.
Dortet et al. (2017)
3 more